Engineering a pH responsive pore forming protein
نویسندگان
چکیده
منابع مشابه
Engineering a pH responsive pore forming protein
Listeriolysin O (LLO) is a cytolysin capable of forming pores in cholesterol-rich lipid membranes of host cells. It is conveniently suited for engineering a pH-governed responsiveness, due to a pH sensor identified in its structure that was shown before to affect its stability. Here we introduced a new level of control of its hemolytic activity by making a variant with hemolytic activity that w...
متن کاملpH dependence of listeriolysin O aggregation and pore-forming ability.
Listeriolysin O (LLO) is the major factor implicated in the escape of Listeria monocytogenes from the phagolysosome. It is the only representative of cholesterol-dependent cytolysins that exhibits pH-dependent activity. Despite intense studies of LLO pH-dependence, this feature of the toxin still remains incompletely explained. Here we used fluorescence and CD spectroscopy to show that the stru...
متن کامل1678 Pore - Forming Protein from E
Entamoeba histolytica, an enter ic h u m a n paras i te , produces extensive tissue des t ruc t ion in the course of the infection. Pr ior studies from this l abo ra to ry have discussed the isolation o f the p l a sma m e m b r a n e of this paras i te and charac te r ized cer ta in o f its intr insic po lypept ides and externa l ly disposed ant igens (1, 2). More recent interests have focused...
متن کاملCharacterization of a membrane pore-forming protein from Entamoeba histolytica
We describe the partial purification and characterization of a pore-forming material (PEM) from Entamoeba histolytica. The formation of ion channels by PFM was examined in three systems. (a) PFM depolarizes J774 macrophages and mouse spleen lymphocytes as measured by [3H]TPP+ uptake. (b) PFM induces rapid monovalent cation flux across the membrane of phosphatidylcholine-cholesterol vesicles. (c...
متن کاملA Novel Composite Membrane for pH Responsive Permeation
Objective(s) In this study, a kind of pH sensitive composite membrane was prepared and drug permeation through it was investigated in terms of pH. Rationale of this study originated from the fact that a pH change which may be a result of a disease state in the body can trigger drug release. Materials and Methods Here, a kind of pH sensitive composite membrane containing different nanoparticle ...
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ژورنال
عنوان ژورنال: Scientific Reports
سال: 2017
ISSN: 2045-2322
DOI: 10.1038/srep42231